Propolis extracts in Juiz de Fora, Minas Gerais: Unveiling quality and identity
DOI:
https://doi.org/10.33448/rsd-v14i3.48517Keywords:
Quality, Bioactive Compounds, Antioxidants, Standardization, Quality Control.Abstract
This research aims to analyze the quality of propolis extracts sold in Juiz de Fora, Minas Gerais, verifying the compliance of these products with the standards established in Normative Instruction N° 3, of January 19, 2001, of the Ministry of Agriculture, Livestock and Food Supply (MAPA). Physicochemical parameters were analyzed, including alcohol content, dry extract, phenolic compounds, flavonoids, wax content and antioxidant activity. The results revealed that two samples presented dry extract below the regulatory limit, while three demonstrated low antioxidant activity, indicating a lower concentration of bioactive compounds. Despite the adequacy of the alcohol and wax contents, the variation in the chemical composition reinforces the influence of factors such as botanical origin and processing on the final quality of the product. These findings highlight the need for more rigorous quality control, ensuring the standardization and safety of the extracts marketed.
References
Aboulghazi A, Touzani S, Fadil M, Lyoussi B (2022) Physicochemical characterization and in vitro evaluation of the antioxidant and anticandidal activities of Moroccan propolis, Veterinary World, 15(2): 341-349. doi.org/10.14202/vetworld.2022.341-349
Bankova, V., Trusheva, B., & Popova, M. (2021). Propolis extraction methods: A review. Journal of apicultural research, 60(5), 734-743. doi.org/10.1080/00218839.2021.1901426
Barreto, A., Lopes, M. D. R., Pereira, F. D. M., & SouZa, B. D. A. (2020). Controle de Qualidade da Própolis.
Brasil. Ministério da Agricultura, Pecuária e do Abastecimento. Instrução Normativa nº 3, de 19 de janeiro de 2001. Aprova os regulamentos Técnicos de Identidade e Qualidade de Apitoxina, Cera de Abelha, Geleia Real, Geleia Real Liofilizada, Pólen Apícola, Própolis e Extrato de Própolis. Brasília, 2001.
Brasil. Ministério da Agricultura, Pecuária e do Abastecimento. Métodos Oficiais para Análise de Produtos de Origem Animal. (1ª ed.), Brasília: MAPA, 2022.
Barrientos‐Lezcano, J. C., Gallo‐Machado, J., Marin‐Palacio, L. D., & Builes, S. (2023). Extraction kinetics and physicochemical characteristics of Colombian propolis. Journal of Food Process Engineering, 46(11), e14272. https://doi.org/10.1111/jfpe.14272
Buitrago, D. M., Perdomo, S. J., Silva, F. A., Cely-Veloza, W., & Lafaurie, G. I. (2024). Physicochemical characterization, antioxidant, and proliferative activity of Colombian propolis extracts: a comparative study. Molecules, 29(7), 1643. doi.org/10.3390/molecules29071643
Campoccia, D., Ravaioli, S., Santi, S., Mariani, V., Santarcangelo, C., De Filippis, A., & Daglia, M. (2021). Exploring the anticancer effects of standardized extracts of poplar-type propolis: In vitro cytotoxicity toward cancer and normal cell lines. Biomedicine & Pharmacotherapy, 141, 111895. doi.org/10.1016/j.biopha.2021.111895
Ferreira, A. R., da Costa Machado, M. T., & Chagas, A. G. C. M. (2023). Propolis oil extract as an alternative means to the alcoholic extract. Observatório De La Economía Latinoamericana, 21(10), 15657-15676. doi.org/10.55905/oelv21n10-063
Gargouri, W., Osés, S. M., Fernández-Muiño, M. A., Sancho, M. T., & Kechaou, N. (2019). Evaluation of bioactive compounds and biological activities of Tunisian propolis. Lwt, 111, 328-336. doi.org/10.1016/j.lwt.2019.05.044
Kawakita, E. T., de Souza, E. A., Uehara, D. M., & de Oliveira Orsi, R. (2015). Avaliação da vida útil do extrato hidroalcoólico de própolis mantido sob diferentes temperaturas de armazenamento. Atas de Saúde Ambiental-ASA (ISSN 2357-7614), 3(1), 33-46.
Laaroussi, H., Ferreira-Santos, P., Genisheva, Z., Bakour, M., Ousaaid, D., Teixeira, J. A., & Lyoussi, B. (2021). Unraveling the chemical composition, antioxidant, α-amylase and α-glucosidase inhibition of Moroccan propolis. Food Bioscience, 42, 101160. doi.org/10.1016/j.fbio.2021.101160
Lima, C. P., Bolino, D. S., Dionísio, K. C., de Miranda, G. S., Maia, G. K. F., de Paula, A. G., & de Campos, G. K. A. (2020). Avaliação da qualidade e atividade antibacteriana de extrato de própolis. Cadernos da Escola de Saúde, 20(1), 29-48. doi.org/10.25192/issn.1984-7041.v20i15318
López, B. G. C., Schmidt, E. M., Eberlin, M. N., & Sawaya, A. C. (2014). Phytochemical markers of different types of red propolis. Food Chemistry, 146, 174-180. doi.org/10.1016/j.foodchem.2013.09.063
Ma, S., Ma, H., Pan, Z., Luo, L., & Weng, L. (2016). Antioxidant activities of propolis’s extracts by different solvents in vitro. Zhongguo Shipin Xuebao, 16, 53-58..
Melo, A. A. M. D., Matsuda, A. H., & Almeida Muradian, L. B. D. (2012). Identidade e qualidade da própolis proveniente de quatro regiões do Brasil. R. Inst. Adolfo Lutz, 540-548. doi.org/10.53393/rial.2012.v71.32462
Mello, B. C., & Hubinger, M. D. (2012). Antioxidant activity and polyphenol contents in Brazilian green propolis extracts prepared with the use of ethanol and water as solvents in different pH values. International Journal of Food Science and Technology, 47(12), 2510-2518. doi.org/10.1111/j.1365-2621.2012.03129.x
Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa científica. Brasil.
Pobiega, K., Kraśniewska, K., & Gniewosz, M. (2019). Application of propolis in antimicrobial and antioxidative protection of food quality–A review. Trends in food science & technology, 83, 53-62. doi.org/10.1016/j.tifs.2018.11.007
R Development Core Team. R: a language and environment for statistical computing. Vienna: R Foundation for Statistical Computing, 2021.
Salatino, A., Fernandes-Silva, C. C., Righi, A. A., & Salatino, M. L. F. (2011). Propolis research and the chemistry of plant products. Natural product reports, 28(5), 925-936. doi.org/10.1039/c0np00072h
Salgueiro, F. B., & Castro, R. N. (2016). Comparação entre a composição química e capacidade antioxidante de diferentes extratos de própolis verde. Química Nova, 39, 1192-1199. doi.org/10.21577/0100-4042.20160136
Silva, S. P., de Carvalho, P. G. S., & Mendes, M. L. M. (2023). Uso da própolis na conservação de frutos: uma revisão sistemática. Observatório De La Economía Latinoamericana, 21(12), 27154-27171. doi.org/10.55905/oelv21n12-198
Shitsuka, R. (2014). Matemática fundamental para tecnologia. Saraiva Educação SA.
Sun, C., Wu, Z., Wang, Z., & Zhang, H. (2015). Effect of ethanol/water solvents on phenolic profiles and antioxidant properties of Beijing propolis extracts. Evidence‐Based Complementary and Alternative Medicine, 2015(1), 595393. /doi.org/10.1155/2015/595393
Wieczorek, P. P., Hudz, N., Yezerska, O., Horčinová-Sedláčková, V., Shanaida, M., Korytniuk, O., & Jasicka-Misiak, I. (2022). Chemical variability and pharmacological potential of propolis as a source for the development of new pharmaceutical products. Molecules, 27(5), 1600. doi.org/10.3390/molecules27051600
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